rabbit anti pard3a Search Results


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a Schemes representing a single auditory HC from the lateral/abneural (top) or medial/neural (bottom) side, and HC orientation (arrows) in the auditory epithelium and the utricular and saccular maculae. In the maculae, two HC populations of opposing orientations are separated by a virtual line of polarity reversal (LPR, yellow dashed line). Domains where HC orientation was quantified in e , f are indicated in blue (utricle: lateral extrastriolar (LES), LPR and medial (M) domains; saccule: anterior (ANT), LPR and posterior (POST) domains). b Phylogenetic tree of class C GPCRs adapted from . c , d LPR region in P2 utricle ( c ) and saccule ( d ). Top panels show a low magnification view with <t>SPTBN2</t> (βII-spectrin) labeling revealing HC orientation by the position of the off-center fonticulus devoid of signal. Bottom panels show a distinct region at higher magnification where PCNT (Pericentrin) labels the basal body below the fonticulus. The LPR can be traced in controls but not in mutants, where all HCs generally point laterally in the utricle and anteriorly in the saccule. e , f Circular histograms of HC orientation by region in the utricle ( e ) and saccule ( f ). Histograms show frequency distribution at P0-P2 (10° bins in a referential where 90° (top) is lateral in the utricle and anterior in the saccule; n indicates HC number in N = 4 animals; Watson U2 test of homogeneity; normalized difference (ND) value indicates how many standard deviations separate the circular means of each distribution). PTXa indicates the Cre-inducible R26-LSL-PTXa allele. Littermate controls for FoxG1-Cre ; PTXa are Cre-negative PTXa animals. Arrows indicate HC orientation. Scale bars are 20 µm ( c , d top), 5 µm ( c , d bottom).
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ECM Biosciences phosphorylated n wasp
a Schemes representing a single auditory HC from the lateral/abneural (top) or medial/neural (bottom) side, and HC orientation (arrows) in the auditory epithelium and the utricular and saccular maculae. In the maculae, two HC populations of opposing orientations are separated by a virtual line of polarity reversal (LPR, yellow dashed line). Domains where HC orientation was quantified in e , f are indicated in blue (utricle: lateral extrastriolar (LES), LPR and medial (M) domains; saccule: anterior (ANT), LPR and posterior (POST) domains). b Phylogenetic tree of class C GPCRs adapted from . c , d LPR region in P2 utricle ( c ) and saccule ( d ). Top panels show a low magnification view with <t>SPTBN2</t> (βII-spectrin) labeling revealing HC orientation by the position of the off-center fonticulus devoid of signal. Bottom panels show a distinct region at higher magnification where PCNT (Pericentrin) labels the basal body below the fonticulus. The LPR can be traced in controls but not in mutants, where all HCs generally point laterally in the utricle and anteriorly in the saccule. e , f Circular histograms of HC orientation by region in the utricle ( e ) and saccule ( f ). Histograms show frequency distribution at P0-P2 (10° bins in a referential where 90° (top) is lateral in the utricle and anterior in the saccule; n indicates HC number in N = 4 animals; Watson U2 test of homogeneity; normalized difference (ND) value indicates how many standard deviations separate the circular means of each distribution). PTXa indicates the Cre-inducible R26-LSL-PTXa allele. Littermate controls for FoxG1-Cre ; PTXa are Cre-negative PTXa animals. Arrows indicate HC orientation. Scale bars are 20 µm ( c , d top), 5 µm ( c , d bottom).
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a Schemes representing a single auditory HC from the lateral/abneural (top) or medial/neural (bottom) side, and HC orientation (arrows) in the auditory epithelium and the utricular and saccular maculae. In the maculae, two HC populations of opposing orientations are separated by a virtual line of polarity reversal (LPR, yellow dashed line). Domains where HC orientation was quantified in e , f are indicated in blue (utricle: lateral extrastriolar (LES), LPR and medial (M) domains; saccule: anterior (ANT), LPR and posterior (POST) domains). b Phylogenetic tree of class C GPCRs adapted from . c , d LPR region in P2 utricle ( c ) and saccule ( d ). Top panels show a low magnification view with <t>SPTBN2</t> (βII-spectrin) labeling revealing HC orientation by the position of the off-center fonticulus devoid of signal. Bottom panels show a distinct region at higher magnification where PCNT (Pericentrin) labels the basal body below the fonticulus. The LPR can be traced in controls but not in mutants, where all HCs generally point laterally in the utricle and anteriorly in the saccule. e , f Circular histograms of HC orientation by region in the utricle ( e ) and saccule ( f ). Histograms show frequency distribution at P0-P2 (10° bins in a referential where 90° (top) is lateral in the utricle and anterior in the saccule; n indicates HC number in N = 4 animals; Watson U2 test of homogeneity; normalized difference (ND) value indicates how many standard deviations separate the circular means of each distribution). PTXa indicates the Cre-inducible R26-LSL-PTXa allele. Littermate controls for FoxG1-Cre ; PTXa are Cre-negative PTXa animals. Arrows indicate HC orientation. Scale bars are 20 µm ( c , d top), 5 µm ( c , d bottom).
Anti Daple Ccdc88c, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology mouse anti acetylated tubulin
a Schemes representing a single auditory HC from the lateral/abneural (top) or medial/neural (bottom) side, and HC orientation (arrows) in the auditory epithelium and the utricular and saccular maculae. In the maculae, two HC populations of opposing orientations are separated by a virtual line of polarity reversal (LPR, yellow dashed line). Domains where HC orientation was quantified in e , f are indicated in blue (utricle: lateral extrastriolar (LES), LPR and medial (M) domains; saccule: anterior (ANT), LPR and posterior (POST) domains). b Phylogenetic tree of class C GPCRs adapted from . c , d LPR region in P2 utricle ( c ) and saccule ( d ). Top panels show a low magnification view with <t>SPTBN2</t> (βII-spectrin) labeling revealing HC orientation by the position of the off-center fonticulus devoid of signal. Bottom panels show a distinct region at higher magnification where PCNT (Pericentrin) labels the basal body below the fonticulus. The LPR can be traced in controls but not in mutants, where all HCs generally point laterally in the utricle and anteriorly in the saccule. e , f Circular histograms of HC orientation by region in the utricle ( e ) and saccule ( f ). Histograms show frequency distribution at P0-P2 (10° bins in a referential where 90° (top) is lateral in the utricle and anterior in the saccule; n indicates HC number in N = 4 animals; Watson U2 test of homogeneity; normalized difference (ND) value indicates how many standard deviations separate the circular means of each distribution). PTXa indicates the Cre-inducible R26-LSL-PTXa allele. Littermate controls for FoxG1-Cre ; PTXa are Cre-negative PTXa animals. Arrows indicate HC orientation. Scale bars are 20 µm ( c , d top), 5 µm ( c , d bottom).
Mouse Anti Acetylated Tubulin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology anti apkc prkcz
a Schemes representing a single auditory HC from the lateral/abneural (top) or medial/neural (bottom) side, and HC orientation (arrows) in the auditory epithelium and the utricular and saccular maculae. In the maculae, two HC populations of opposing orientations are separated by a virtual line of polarity reversal (LPR, yellow dashed line). Domains where HC orientation was quantified in e , f are indicated in blue (utricle: lateral extrastriolar (LES), LPR and medial (M) domains; saccule: anterior (ANT), LPR and posterior (POST) domains). b Phylogenetic tree of class C GPCRs adapted from . c , d LPR region in P2 utricle ( c ) and saccule ( d ). Top panels show a low magnification view with <t>SPTBN2</t> (βII-spectrin) labeling revealing HC orientation by the position of the off-center fonticulus devoid of signal. Bottom panels show a distinct region at higher magnification where PCNT (Pericentrin) labels the basal body below the fonticulus. The LPR can be traced in controls but not in mutants, where all HCs generally point laterally in the utricle and anteriorly in the saccule. e , f Circular histograms of HC orientation by region in the utricle ( e ) and saccule ( f ). Histograms show frequency distribution at P0-P2 (10° bins in a referential where 90° (top) is lateral in the utricle and anterior in the saccule; n indicates HC number in N = 4 animals; Watson U2 test of homogeneity; normalized difference (ND) value indicates how many standard deviations separate the circular means of each distribution). PTXa indicates the Cre-inducible R26-LSL-PTXa allele. Littermate controls for FoxG1-Cre ; PTXa are Cre-negative PTXa animals. Arrows indicate HC orientation. Scale bars are 20 µm ( c , d top), 5 µm ( c , d bottom).
Anti Apkc Prkcz, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a Schemes representing a single auditory HC from the lateral/abneural (top) or medial/neural (bottom) side, and HC orientation (arrows) in the auditory epithelium and the utricular and saccular maculae. In the maculae, two HC populations of opposing orientations are separated by a virtual line of polarity reversal (LPR, yellow dashed line). Domains where HC orientation was quantified in e , f are indicated in blue (utricle: lateral extrastriolar (LES), LPR and medial (M) domains; saccule: anterior (ANT), LPR and posterior (POST) domains). b Phylogenetic tree of class C GPCRs adapted from . c , d LPR region in P2 utricle ( c ) and saccule ( d ). Top panels show a low magnification view with SPTBN2 (βII-spectrin) labeling revealing HC orientation by the position of the off-center fonticulus devoid of signal. Bottom panels show a distinct region at higher magnification where PCNT (Pericentrin) labels the basal body below the fonticulus. The LPR can be traced in controls but not in mutants, where all HCs generally point laterally in the utricle and anteriorly in the saccule. e , f Circular histograms of HC orientation by region in the utricle ( e ) and saccule ( f ). Histograms show frequency distribution at P0-P2 (10° bins in a referential where 90° (top) is lateral in the utricle and anterior in the saccule; n indicates HC number in N = 4 animals; Watson U2 test of homogeneity; normalized difference (ND) value indicates how many standard deviations separate the circular means of each distribution). PTXa indicates the Cre-inducible R26-LSL-PTXa allele. Littermate controls for FoxG1-Cre ; PTXa are Cre-negative PTXa animals. Arrows indicate HC orientation. Scale bars are 20 µm ( c , d top), 5 µm ( c , d bottom).

Journal: Nature Communications

Article Title: EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia

doi: 10.1038/s41467-021-22997-1

Figure Lengend Snippet: a Schemes representing a single auditory HC from the lateral/abneural (top) or medial/neural (bottom) side, and HC orientation (arrows) in the auditory epithelium and the utricular and saccular maculae. In the maculae, two HC populations of opposing orientations are separated by a virtual line of polarity reversal (LPR, yellow dashed line). Domains where HC orientation was quantified in e , f are indicated in blue (utricle: lateral extrastriolar (LES), LPR and medial (M) domains; saccule: anterior (ANT), LPR and posterior (POST) domains). b Phylogenetic tree of class C GPCRs adapted from . c , d LPR region in P2 utricle ( c ) and saccule ( d ). Top panels show a low magnification view with SPTBN2 (βII-spectrin) labeling revealing HC orientation by the position of the off-center fonticulus devoid of signal. Bottom panels show a distinct region at higher magnification where PCNT (Pericentrin) labels the basal body below the fonticulus. The LPR can be traced in controls but not in mutants, where all HCs generally point laterally in the utricle and anteriorly in the saccule. e , f Circular histograms of HC orientation by region in the utricle ( e ) and saccule ( f ). Histograms show frequency distribution at P0-P2 (10° bins in a referential where 90° (top) is lateral in the utricle and anterior in the saccule; n indicates HC number in N = 4 animals; Watson U2 test of homogeneity; normalized difference (ND) value indicates how many standard deviations separate the circular means of each distribution). PTXa indicates the Cre-inducible R26-LSL-PTXa allele. Littermate controls for FoxG1-Cre ; PTXa are Cre-negative PTXa animals. Arrows indicate HC orientation. Scale bars are 20 µm ( c , d top), 5 µm ( c , d bottom).

Article Snippet: Primary antibodies used were: goat anti-GPR156 (Santa Cruz; sc102572; TCA; 1:100), rabbit anti-GPR156 (Novus; NBP1-83402; TCA; 1:100), mouse anti-acetylated Tubulin (Santa Cruz; 23950; PFA; 1:500), rabbit anti-pericentrin/PCNT (Biolegend; PRB-432C; PFA; 1:400), mouse anti-βII-Spectrin/SPTBN2 (BD Transduction Lab; 612562, PFA; 1:200), rat anti-ZO1 (Developmental Studies Hybridoma Bank; R26.4C; TCA; 1:200), rabbit anti-Gαi3 (Santa Cruz; sc-262; PFA; 1:400), chicken anti-Gαi3 (Sigma; GW22489; PFA; 1:400; used for cochlear explants), rabbit anti-DAPLE/CCDC88C (Proteintech; 25769-1-AP; TCA; 1:400), mouse anti-MYO7A (Developmental Studies Hybridoma Bank; 138-1; PFA; 1:500), goat anti-FZD6 (R&D Systems; AF1526; PFA; 1:200), rabbit anti-VANGL2 (gift from Philippe Gros, McGill University; PFA; 1:500), goat anti-GPSM2/LGN (ThermoFisher Scientific; PA5-18646; PFA; 1:200), rabbit anti-βGalactosidase (Cappel discontinued aliquot; PFA; 1:1000; now MP Biomedical 55976), mouse anti-aPKC/PRKCZ (Santa Cruz; sc-216; PFA; 1:100), rabbit anti-PARD6B (Santa Cruz; sc-67393; PFA; 1:100), rabbit anti-PARD3A (Proteintech; 11085-1-AP; PFA; 1:200), rabbit anti-CALB1 (Cedarlane/Millipore; AB1778(CH); PFA; 1:500), goat anti-SPP1/Osteopontin (R&D Systems; AF808; PFA; 1:100), goat anti-SOX2 (Santa Cruz; 17320; PFA; 1:500), rabbit anti-EMX2 (Trans Genic; KO609; PFA; 1:250).

Techniques: Labeling